![]() Powered by Next GEM technology, the Chromium Controller enables integrated analysis of single cells at massive scale. Uncover critical cellular insights with optimized workflows that streamline your studies and expand sample access to include fresh, PFA-fixed, and FFPE tissues. Chromium NextGEM Secondary Holder 10x Magnetic Separator Eppendorf Master Cycler Nexus Flexlid Mini Centrifuge (for 8-tube strip, and for 1.5ml Eppendorf). The Chromium Controller uses advanced microfluidics to perform single cell partitioning and barcoding in a matter of minutes. From translational immunology and immuno-oncology, to infectious disease research-these technological advancements, along with our intuitive software analysis and visualization tools, will accelerate the understanding of the adaptive immune system.Chromium Single Cell Gene Expression Flex provides our most sensitive whole transcriptome profiling with ultimate flexibility, making single cell analysis possible where it wasn’t before.A comprehensive approach to simultaneously examine the cellular context of the adaptive immune response and the immune repertoires of hundreds to millions of T and B cells on a cell-by-cell basis.The Chromium Single cell Immune Profiling Solution Encapsulate up to 80,000 cells in less than 10 minutes 10x Genomics’ single cell RNA-seq (scRNA-seq) technology, the Chromium Single Cell 3’ solution, allows you to analyze transcriptomes on a cell-by-cell basis through the use of microfluidic partitioning to capture single cells and prepare barcoded, next-generation sequencing (NGS) cDNA libraries.More efficient than leading academic droplet systems Click to TOC Chromium Next GEM Single Cell 3 Reagent Kits v3.1 User Guide with Feature Barcoding technology for Cell Surface Protein Rev D 7 Chromium Next GEM Single Cell 3 Library Kit v3.1 16 rxns PN-1000157 (store at 20☌) Chromium Next GEM Single Cell 3 GEM, Library & Gel Bead Kit v3.Complete practical solution for single cell analysis Identify rare cell types in heterogeneous populations.High-throughput single cell transcriptomic measurements enable discovery of gene expression dynamics for profiling individual cell types.Single Cell 5 Library Kit (10X, PN-1000165) workflow, or the Chromium Next GEM Single Cell. Key advantages of the Chromium Single cell RNA-Seq 10X Genomics Chromium Controller System: Cells or nuclei are. There are approximately 80,000-100,000 total GEMs after running Chip A in the Chromium Controller, and 1-10 of GEMs contain cells depending on the total number of cells loaded. Finally, the Chromium™ Software Suite is utilized for processing, analysis and visualization of single cell gene expression data. ![]() 2) As part of our warranty policy, clog and wetting failures that have been properly documented are reimbursed with replacement reagents and chips. High-quality next generation sequencing libraries are finished in single bulk reaction. Recommendations: 1) If a wetting failure occurs during GEM generation, we recommend re-preparing the sample. ![]() Library construction using GEM is performed using Chromium controller. Lysis and barcoded reverse transcription of polyadenylated mRNA from single cells are performed inside each GEM. New horizons for Next-Generation Sequencing with GemCode technology (10x Genomics). Single cells, reagents and a single Gel Bead containing barcoded oligonucleotides are encapsulated into nanoliter-sized GEMs (Gel Bead in emulsion) using the GemCode Technology. The Chromium™ Single Cell RNA sequencing Solution The 10X Genomics Chromium Single Cell 3 GEM Library and Gel Bead Kit v3 enables simultaneous library preparation of hundreds to thousands of individual. The GemCode™ Technology from 10X Genomics, which combines microfluidics with molecular barcoding and custom bioinformatics software enable analyzing the transcriptomes of thousands of cells at single cell resolution. Advances in single cell RNA quantification techniques have enabled comprehensive study of cell subpopulations within a heterogeneous population. Figure 2 The Chromium DNA partitioning workflow for genome sequencing.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |